TY - BOOK AU - Mishra, Vikas, editor TI - Genetic engineering: principles and advancements AV - EBOP QH 442 G46 2019 PY - 2019/// CY - Oakville, Ontario PB - Delve Publishing KW - Genetic engineering N1 - SECTION I GENETIC ENGINEERING IN ANIMALS Chapter 1: Birth of Clones of The World’s First Cloned Dog Chapter 2: Generation of Cloned Mice and Nuclear Transfer Embryonic Stem Cell Lines From Urine-Derived Cells Chapter 3: A One-Step Cloning Method For The Construction of Somatic Cell Gene Targeting Vectors: Application to Production of Human Knockout Cell Lines Chapter 4: Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination SECTION II GENETIC ENGINEERING IN PLANTS Chapter 5: A Modified Gateway Cloning Strategy for Overexpressing Tagged Proteins in Plants Chapter 6: A Simple, Flexible and Efficient PCR-Fusion/Gateway Cloning Procedure For Gene Fusion, Site-Directed Mutagenesis, Short Sequence Insertion and Domain Deletions and Swaps Chapter 7: Colorful-Circuit: A Platform For Rapid Multigene Assembly, Delivery, And Expression In Plants Chapter 8: CRISPR-Based Tools For Plant Genome Engineering SECTION III GENETIC ENGINEERING IN DROSOPHILA Chapter 9: Single Vector Non-Leaky Gene Expression System For Drosophila Melanogaster Chapter 10: Systematic Comparison of 2A Peptides For Cloning Multi-Genes in a Polycistronic Vector Chapter 11: An Entry/Gateway® Cloning System For General Expression of Genes With Molecular Tags In Drosophila Melanogaster Chapter 12: Gene Tagging Strategies To Assess Protein Expression, Localization, And Function In Drosophila Chapter 13: Damage To The Drosophila Follicle Cell Epithelium Produces “False Clones” With Apparent Polarity Phenotypes Chapter 14: Dm5-HT2B: Pharmacological Characterization Of The Fifth Serotonin Receptor Subtype Of Drosophila Melanogaster N2 - Genetic Engineering: Principles and Advancements considers various aspects of genetic engineering including an extensive overview of genetic engineering and related principles and advancements. It includes Molecular Biology and Biotechnology Birth of clones of the world’s first cloned dog, generation of cloned mice and nuclear transfer embryonic stem cell lines from urine-derived cells, a one-step cloning method for the construction of somatic cell gene targeting vectors: application to production of human knockout cell lines. Provides the reader with insights into the development of its history, so as to understand the Dm5-HT2B: Pharmacological Characterization of the Fifth Serotonin Receptor Subtype of Drosophila melanogaster, Extensive cross-regulation of post-transcriptional regulatory networks in Drosophila ER -